Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs

Skeletal muscle growth in livestock impacts meat quantity and quality. Concerns arise because certain feed additives, like beta-agonists, may affect food safety. is a specialized tissue consisting of nondividing multinucleated fibers. Myostatin (MSTN), protein specific to skeletal muscle, secreted functions as negative regulator mass by inhibiting the proliferation differentiation myoblasts. To enhance growth, phytogenic additives could be an alternative they inhibit MSTN activity. The objective this study was establish systematic screening platform using activity evaluate phytogenics, providing scientific evidence their assessment potency. In study, we established monitor myostatin promoter rat L8 Extract Glycyrrhiza uralensis (GUE), oriental herbal medicine, identified through platform, active fractions GUE were process-scale liquid column chromatography system. For vivo additive investigated growth-finishing pigs. results showed that significantly increased body weight, carcass lean content Microbiota analysis indicated did not composition gut microbiota summary, rodent myoblast used identify myogenesis-related phytogenic, GUE, further demonstrated compounds inhibited expression. These findings suggest novel application for performance enhancement modulation Moreover, well-established holds significant potential identifying assessing diverse range phytogenics contribute process myogenesis.